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 When it comes to low temperature requirements, 
        what are the current limits of environmental test chambers? 
      
        - 20°C above ambient air — this often the lowest 
          achievable temperature in a chamber that has no built-in refrigeration 
          system. This is not a very reliable specification because the chamber's 
          components (such as the motor of the re-circulating fan) can generate 
          additional heat. This low temperature is usually specified for environmental 
          ovens.
   
         
        - 5 to -20°C — the lowest achievable temperature 
          using standard R-134A refrigerant. This is the same stuff used in new 
          air conditioning systems and refrigerators. Because of its limited range, 
          it's not widely used in test chambers.
 
           
         
        - -35 to –40°C — the lowest range achiveable 
          in chambers using R-404A or R-507 refrigerants. The system that uses 
          these refrigerants is sometimes called "single stage" (i.e., 
          one compressor).
 
           
         
        - -65 to –73°C — this low-temperature range is 
          achievable via "cascade" refrigeration: a "high-stage" 
          refrigeration circuit is used to cool the "low-stage" circuit. 
          Cascade cooling is not very efficient, so the actual "cooling" 
          samples being tested is slower. Because the air is much cooler, it may 
          appear that the chamber is working fat. In actuality, it takes 
          the samples longer to catch up. 
 
           
         
        - -85 to -150°C — these temperatures are achieved 
          using a triple-cascade refrigeration system. This is not common in test 
          chambers, although it's frequently used in ultra-cool freezers. 
 
           
         
        - -170°C — achieved using use liquid nitrogen. 
          Nitrogen is an inexpensive method for cooling a chamber; however, its 
          long-term use of is expensive. Liquid-nitrogen refigeration systes are 
          best for: Small or not-often-used chambers; Chambers that specifically 
          need this low range; and Chambers that need temperature change rates 
          greater than 30°C/min.
 
       
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